THE WITWATERSRAND DEEP MICROBIOLOGY PROJECT:PRIVATE SITE

Aqueous and Isotope Geochemistry and 16SrDNA



dD versus d18O for fissure water, service water, precipitation and hotsprings and Vaal river (courtesy of Princeton University and University of Toronto)

Br versus Cl for fissure water and service water (courtesy of Princeton University)


Ca versus Na for fissure water and service water (courtesy of Princeton University)

The tables below list 16SrDNA and enrichment data available for water samples indicated in the figures above and for rock, biofilm and air samples.
SampleName
Bacterial 16SrDNA
Archaeal 16SrDNA
Enrichments
Flow Cyto Biomass (cells/ml)
PLFA Biomass
(cells/ml)
Water Samples
BE(1)16FW 031401IDW
Gihring
Gihring
  
43,000
  
BE(1)16FW 031601GDW hole 1
Lin, Esta
Lin
  
6,600
  
BE116FW 121801CXN
PU
PU
NMIT
  
UT
BE(2)23FW 031301 A4RD 
Lin
-
  
136,000
UT
BE(2)24FW 032601 C18W17
Lin
-
  
<5000
  
BE(2)23SW 031301
Lin
Lin
      
B(E)225FW1 
KTDPM
KT
    
50,000
BE339FW 032701CTS hole 1
Lin
-
  
19,000
N.A.
BE341FW 032701CTS hole 3
Lin
-
  
<5000
N.A.
(DR)E4IPC (dolomite)
KTDPM
KT
Thiosulfate and S oxidizers
  
25,000
DR4IPC 082902-massive filter
 PU
PU 
  
 PU
  
(DR)  E546bh1 
-
    
310,000
DR546BH1 103001
 +
-
 
 PU
 UT
DR546SW 103001
PNNL
PNNL 
    
UT
(DR546) E5-sump    
Alkaphilus Transvaalensis
  
175,000
(DR546) EDS-1 (service water) 080198
KT
KT
    
1,250,000
DR5 46 SW2 (service water) 120198
DPM
-
DR546BH1 110802
DR548 ceiling weep 121598
Bacillus Thermoleovorans
DR5 E5030 101502 Stope water
Moser&Gihring
Moser&Gihring
DR5 E5030xCUT 101502 Stope sludge
Moser&Gihring
Moser&Gihring
DR5 E5030x CUT 101502 Acid seep water
Moser&Gihring
Moser&Gihring
DR5 E50 101502 Service Water
Moser&Gihring
Moser&Gihring
(DR638) WDF1 (acid seep)  
KTDPM, KT
N.A.
N.A.
12,500
(DR638) WDF2b
KTDPM
KTDPM, KT
N.A.
N.A.
250
(DR638)W638bh1
Baker
Baker
McCuddy
N.A.
N.A.
DR9 IPC 101602 H1-massive filter
DR938 FLOOR DRAIN 110201
PNNL
PNNL
N.A.
N.A.
N.A.
DR938FW082001 hole 1
PU
PU
  
PU
UT
DR938 H1 110201
PNNL
PNNL
N.A.
PU
N.A.
DR938 H3 0M 102401
PNNL
PNNL
N.A.
PU
N.A.
DR938 H3 110701-dream borehole
 +
+
IRB, SRB, So
N.A.
UT
DR938 H3 125M 102401&102501
PNNL
PNNL
    
UT
DR938 H3 250M 102401&102501
PNNL
PNNL
    
UT
DR938 H3 390M 102401
PNNL
PNNL
    
UT
DR938 H3 550M 102501
PNNL
PNNL
    
UT
DR938 H3 648M 102501
PNNL
PNNL
    
UT
DR938CH 110701-six pack
+
PNNL
    
UT
DR938CH (WD9386PK) 071102-six pack
PU
PU
.
PU
UT
DR938CH 091602-massive flter
Moser&Gihring
Moser&Gihring
DR938CH 102102
Moser&Gihring
Moser&Gihring
DR938 H3 (H2) 071202-massive filter
Trimarco
PU
  
PU
UT
DR938 H3 111702
Moser&Gihring
Moser&Gihring
DR940 092602
EV219FW 030901ED hole 5
Mislowack
Mislowack
Aeromonas Hydrophylia
<5000
  
EV219SW 030901
PNNL
PNNL
       
EV219FW081601 hole 3
PU
PU
   
PU
UT
EV219FW082902 hole 5 MeOH
 Meth Bacterial Sequences & relatives
 Meth Arch sequences & relatives
EV219FW082902 hole 5 IRB
 IRB Bact relatives and sequences
 IRB Arch relatives and sequences
EV219FW082902 hole 5 SRB
 SRB cart Arch seq & relatives
EV219FW082902 hole 5 Control
 Bacterial clones &relatives-control
 Archeal clones & relatives-control
EV221 BH1 111601
PNNL
PNNL
      
EV221 BH2 111601-acidic
+
+
  
PU
UT
EV221 BH3 111601-six pack
PNNL
PNNL
  
PU
UT
EV221 SW  111601
PNNL
PNNL
    
UT
EV221FW020502FWD27-BH1
PU
PU
      
EV2(21) 7C21 103002
EV221 CH29 103002
EV221 SW 103002 service water
EV221 BH1 121702 Group 2
EV221 BH29 121702 Group 1
EV224SW081302-first coring site
PU
PU
N.A.
N.A.
UT
EV522FW030801HWD
+, but not sequenced
+, but not sequenced
N.A.
<5000
UT
EV522FW030801HWD
PU
PU
N.A.
160,000
UT
EV818FW030601NEPD
 Davidson
-
N.A
10,700
N.A.
EV818SW030601
PNNL
PNNL
N.A.
11,000
N.A.
EV818FW051001NEPD
Davidson
Davidson
Kieft-Desulfovibrio
63,000
UT
EV818FW 062101FBH
Davidson
Davidson
McCuddy
54,000
UT
EV818BHEB5 DRLW 102502
EV818BHE6 DRLW-sludge 102502
EV818BHEB5 102502
EV818SW 102502
EV818 BEHB5 102702
EV818 BEHB6 102702
EV818 BEHB6 111502
EV818 BEH6A 121302
EV820FW121401
PU
PU
N.A.
N.A.
UT
EV820061802 20NWD-mineral cavity
PU
PU
N.A.
PU
UT
EV821FW101601
+, but not sequenced
+, but not sequenced
N.A.
N.A.
UT
EV821SW101601
PNNL
PNNL
N.A.
N.A.
UT
EV914dFW 022801ED
 Esta
Lin
N.A
2,400,000
N.A.
K(L) 441SW
KTDPM
KTDPM,KT
N.A.
N.A.
6,600
K(L) 441FW1
KTDPM
KTDPM, KT
N.A.
N.A.
2,200
K(L) 441FW1b
KTDPM
-
N.A.
N.A.
1,700
KL441SW 022801 
Hall
Hall
N.A.
N.A.
16,000
KL441FW 022801HWDN
Hall
Lin
N.A.
<5000
UT
KL441FW 050201XC56HWDS H2 
N.A.
N.A.
Kieft
<5000
500
KL441FW 051702-XC56HWDS H2
PU
PU
N.A.
N.A.
N.A.
KL441FW 100201XC56HWDS H2
+, but not sequenced
<d.l.
N.A.
N.A.
1500
KL441FW 111401XC56HWDS  H3
+, but not sequenced
+, but not sequenced
N.A.
PU
DEAD
KL441DB 02280153EXC 
PU
PU
N.A.
20,000,000
N.A.
KL443FW 050801XC43HWDN
PU
PU
N.A.
<5000
5600
KL443FW 071301
McCuddy
KL739 062901
+
-
McCuddy
10,000
933
MM5-1870FW 030402SW haulage
Bonin
Bonin
Bonin
PU
UT
MBNWFW 040301Sec49-Karoo coal
methanogen sequence
+, but not sequenced
  
PU
  
MPI-49FW 020802XCTH3 (Merriespruit)
Bonin
Bonin
Bonin
    

 
 
SampleName
Bacterial 16SrDNA
Archaeal 16SrDNA
Enrichments
Flow Cyto Biomass (cells/ml)
PLFA Biomass
(cells/ml)
Water Samples
MPI-51FW022202XCT8EAST (Merriespruit) Bonin Bonin PU UT
(Mponeng) WUD109
MP104E65XC 091602
MP104E65XC 091902-massive filter
MP104E65XCBH1 093002-massive filter
MP104E65XC 110902
NO27RW100501 SW1
Moser&Gihring
Moser&Gihring
NO27RWFWDE100501 Hose 1
+
+
PR763 090501 hole 3
McCuddy
TT100X/CS062802P2-massive filter
Rock or Core Samples
DR5 46/48 HS3i
+
-
DR5 46/48 3-3CLi
+
-
EV224 081302 core 1
EV224 081302 core 2
EV818 BEHB6 102702 core 1
Moser&Gihring
Moser&Gihring
EV818 BEHB6 102702 core 2
Moser&Gihring
Moser&Gihring
Biofilm Samples
DR5 46 
EV219 
DR5 E5030xCUT 101502
Moser&Gihring
Moser&Gihring
DR938H3 111702 Floor 
Moser&Gihring
Moser&Gihring
EV818 BEH6A 121302
EV221 BH29 121702 Group 1
Air Samples
DR5 46 Air
+
+
EV818 BEH6A 121302
EV221 BH29 121702 Group 1