THE WITWATERSRAND DEEP MICROBIOLOGY PROJECT: PRIVATE SITE

Cross Section of Kloof Mine


(Figure from van Heerden, 2001)



  Simplified geological cross section of the Kloof mining property, west Wits line, showing levels 39, 41 and 43.  The youngest stratagraphic formation is the 2.4 Ga Pretoria group siliclastic sediments and diabase sills (fed by the Running Dyke?).  This overlies the 2.5 Ga Malmani Dolomite which is a principal aquifer in the region and at Libanon is unconfined, but at 4 shaft is confined.  The dolomite overlies the 2.7 Ga Ventersdorp volcanics and the contact is marked by the "Black Reef".   The Ventersdorp volcanics overlie the 2.9 Ga Central Rand Group of the Witwatersrand Supergroup and the contact is marked by the VCR.  The Central Rand contain the MR and the Carbon leader.  All of the deeper levels at 4 shaft mine the VCR, but 39 level also mines the CL of the Central Rand Group.  Shaft 7 of Kloof is just a few kilometers west of the 4 shaft and a sample was collected from the 39 level there, presumably at about the same depth as 39 level in 4 shaft.  The strata are partitioned into "compartments" by dykes of varying ages.  The 1.4 Ga syenite dikes radiating from the Pilansberg Complex (within the Bushveld Complex) to the north cut all formations (Venterspost and Kloof dykes). Water from the dolomite enters into the underlying Witwatersrand beds primarily through fissures arranged en echelon to these dike aquicludes.  Running dyke must be ca. Transvaal in age or perhaps 2.0 Ga?  The dykes associated with Danies Fault and perhaps the Glenharvie dykes are Ventersdrop in age as they are all truncated at the contact with the overlying dolomites.  Faults are often associated with dykes and vice-versa dykes will follow faults (any weakness in the crust).  Finally, the Witpoortjie Fault truncates the Wits strata and may be a 2.0 Ga fault.  If so it should come to surface ca. 10 km south of Glenharvie.  If this fault separates basement gneiss from the Wits strata, then it may act as a conduit for deeper fluids that may feed the subvertical fractures associated with the dykes.
 
 

Map of 41 to 45 Levels of 4 Shaft Complex-Kloof Mine


(Figure from van Heerden, 2001)

The biggest water intersection in mine history-41 Level 56 Hanging Wall Drive South

The very first set of samples collected from Kloof were taken from 41 level 56 hanging wall drive south (K441 FW1 and 1a) and from 41 level 53 hanging wall drive north (K441FW2).  More recently, these sites have been resampled.  Danies fault zone was also sampled recently at 43 level.
 




    Samples KL441FW56HWDS come from fractures adjacent to the Glenharive Dyke.  When first encountered while cover drilling during tunnel advancement several months prior to our first sampling, the flow rates were 100,000 liters/hour.  It took a week just to put a valve on this one borehole.  Several other boreholes were drilled through cased valves and more water encountered.  A tracer test was performed using a fluorescent dye to test the connectivity of different boreholes.  The fractures believed to be responsible for the water are illustrated as dashed blue lines beyond the tunnel face.  Approximately 7 tons of chemically treated concrete were injected into these holes prior to our first sampling (blue boreholes) in '98.  Subsequent to sampling the head of the tunnel was advance another 3 meters.  No further advancement is planned.  Ventilation is generally shut off now.  Subsequent samples have come from other boreholes (red).

Kloof-Aqueous Geochemistry, Dissolved Gas and Microbial Results

(courtesy of Princeton University, University of Toronto, New Mexico Tech. and Univ. of Witswatersrand)
 
Kloof-Aqueous Geochemistry Results

The tables below list data available for samples of fissure water collected from Kloof mine, service water collected from Kloof mine and dolomite water collected from the IPC chamber  from shaft 4 of Driefontein mine.  The analyses are ordered from right to left according to the salinity, with the fresh intersection at 4 shaft Kloof, 43 level being the most saline (approximately 2% wt).  

The data include field measurements of pH, pe, dissolved O2, temperature, conductivity, and water and gas flow rates. The number of microbial cells, as detected by flow cytometry (detection limit <5 x 103 cells/ml), are reported.  The types of microorganisms that have been successfully cultivated in the laboratory at room temperature (viewed as contaminants with the exception of the dolomite water), 60oC (thermophiles) and 80oC (hyperthermophiles).  SRB are sulfate reducing bacteria.  Het. are heterotrophic (organic carbon utilizers).  Me are methanogens.  IRB are Fe(III) reducing bacteria.  Sox are sulfide oxidizing bacteria.  Thio. are thiosulfate oxidizing bacteria.  The TOC is total organic carbon , followed by specific carboxylic acids commonly utilized by bacteria.  CO2 represents the total inorganic carbon.  The organic and inorganic carbon species are still being determined for all Kloof samples.  These analyses are followed by the anions, major cations and the trace metals.  The last part of the table represents the calculated saturation levels, of S.I. of various minerals.  S.I. = 0 means the mineral is at saturation. S.I. > 0 means the mineral phase will form a precipitate.  S.I. < 0 means the mineral phase will dissolve.  

Fissure water is most readily distinguished from service water by the relative proportions of anions. Service water is low in chloride relative to bromide, high in sulfate (due to sulfide oxidation) and high in nitrate (due to explosives).  Fissure water is largely  comprised of Na, Ca and Cl.  Fissure water is typically saturated with respect to calcite and this is consistent with observations of calcite filling water-bearing fractures.  Fissure water typically contains no dissolved O2, with the exception of samples collected from shaft 4, level 41, hanging wall drive south.  This presence of O2 in the samples may either indicate that this is a hybrid mixture of surface water and deep fissure water, or it may reflect the difficulty in packing off the boreholes at this locality.  Air contamination can also be seen in the gas samples (see Sherwood-Lollar data).  We will be returning to this location again to with packers designed to match borehole sizes in order to test whether the water is aerobic or anaerobic.   Samples associated with Danies fault, however, are demonstrably anaerobic.  The most saline , anaerobic fissure water do yield thermophilic and hyperthermophilic microorganisms, but do not yield room temperature microorganisms.  This indicates the precautions taken in the field and lab to avoid contamination have been largely successful.  


 

SampleName
IPC#4 120900
KL441SW 022801 
KL441FW 050201 XC56HWDS hole 1 
KL441FW 050201 XC56HWDS hole 2 
KL441FW 022801 HWDN 
KL441DB 022801 53EXC 
KL739FW 062901
KL443FW 050801 XC43 HWDN
Depth (kmbls.)
0.945
3.3
3.3
3.3
3.3
3.3
3.1?
3.44?
pH
7.5
N.A.
12.5
8.4
8.7
5.5
5.8
8.2
Eh (mv)
N.A.
N.A.
N.A.
-260
N.A.
N.A.
-220
-300
pe
 
 
 
-3.98 (-5.4)
 
 
-3.39
-4.55 (-5.13)
Cond. (mS/cm)
N.A.
N.A.
6.8
5.3
N.A.
N.A.
N.A.
25 at RT
ToC
26
N.A.
50
56
49
N.A.
54
59
O2 (ppm)
0
N.A.
3
0
2
N.A.
0
0
Alkalinity (ppm)
>100
N.A.
>100
>100
N.A.
N.A.
N.A.
12
Water Flow Rate (ml/min)
 
N.A.
725
17,650
1000
3.5 to 4.0
9,000
22,200
Gas Flow Rate (ml/min)
 
N.A.
nil
214
nondetected
none
10,000
2,600
Microbial Properties
 
 
 
 
 
 
 
 
Direct Counts 04/01 (cells/ml)
?
N.A.
 
?
?
2e7
?
?
Direct Counts 08/01(cells/ml)
 
N.A.
 
<5e3
<5e3
 
1e4?
<5e3
MPN's (cells/ml)
>1e2
N.A.
 
>1
 
 
>1
>1
Positive Enrichments 80oC
N.A.
N.A.
 
Me33, Dil. Het.27, 32,  IRB
N.A.
 
 
Dil. Het.24,29, SRB
Positive Enrichments 60oC
N.A.
N.A.
 
Me9, SRB14, IRB12
N.A.
 
SRB, Thio.
Me10, SRB8, Dil. Het.16
Positive Enrichments Room T
Sox, Thio.
N.A.
 
IRB21
N.A.
 
 
 
Anions & Cations (ppm)
 
 
 
 
 
 
 
 
TOC
29
N.A.
130.0 
5.5 
N.A.
N.A.
5.9 
20.0 
acetate
 
 
 
 
 
 
 
 
propionate
 
 
 
 
 
 
 
 
formate
 
 
 
 
 
 
 
 
CO2 Total (Chemnet)
>100
N.A.
<10
<10
N.A.
N.A.
N.A.
<10
HCO3-
 
 
 
 
 
 
 
 
F
0.44
N.D.
0.11
0.48
1.35
N.D.
1.53
3.75
Cl
14.01
197.45
1,221.37
1,563.32
5,073.16
7,609.34
8,184.92
12,494.88
Br
N.D.
11.92
6.18
7.5
15.78
37.78
47.04
45.91
SO42-
71.38
669.54
30.91
6.14
50.74
79.03
6.27
149.2
HS- (Chemnet)
<0.1
N.A.
0
0.4
0.4
N.A.
N.A.
1.5
NO3-
N.D.
18.27
0.06
N.D.
0.13
N.D.
N.D.
0.01
NO2-
N.D.
N.D.
N.D.
N.D.
0.26
0.27
N.D.
N.D.
NH3 (Chemnet)
<.1
N.A.
0
0.2
N.A.
N.A.
3
5
PO43-
N.D.
N.D.
0.05
0.03
N.D.
N.D.
N.D.
N.D.
Total P as PO43-
1.291
1.08
3
4.2
0.913
9.05
4.65
4.3
Li  
N.D.
0.12
0.421
0.423
1.9
2.2
1.36
1.84
Na 
18.20
157.00
384.00
452.00
1,865.00
2,025.00
1,680.00
2,580.00
Mg 
42.6
39.35
N.D.
N.D.
4.75
4.22
0.15
0.105
2.2
11.6
13.9
8.69
33.9
37.1
41.4
52.2
Rb
N.A.
N.A.
0.121
0.114
N.A.
N.A.
0.173
0.248
Ca 
56.25
250.00
595.00
458.00
1,965.00
2,122.40
2,800.00
3,280.00
Sr 
0.1
1.465
7.8
8.5
39.2
44.4
90
46.3
Ba 
0.11
0.049
0.26
0.038
0.8
0.835
2.09
0.581
Al 
1.37
0.83
0.561
0.048
0.655
0.88
0.36
0.091
Si 
11.2
13.8
1.16
18.9
16.9
17.6
12.2
11.3
Mn 
0.06
0.11
N.D.
0.001
0.036
N.D.
0.0065
0.011
Fe 
0.53
0.407
0.005
0.0035
1.466
0.556
0.01
0.005
Fe(II)  (Chemnet)
N.A.
N.A.
0.1
0
0
N.A.
<1
0
FeTotal (Chemnet)
N.A.
N.A.
0.2
0.2
1
N.A.
N.A.
0
Cr 
N.D.
N.D.
0.02
0.02
N.D.
0.03
0.016
0.022
Co
N.D.
0.07
0.0015
0.001
N.D.
N.D.
0.0025
0.0025
Ni 
0.015
0.5
0.0125
0.0115
N.D.
N.D.
0.04
0.027
Cu
N.D.
N.D.
0.01
0.004
N.D.
N.D.
0.009
0.025
Zn 
0.03
0.51
0.111
0.015
1.8
N.D.
0.019
0.03
As 
N.D.
N.D.
0.08
0.087
N.D.
N.D.
0.011
0.105
N.D.
N.D.
 
 
N.D.
N.D.
 
 
U
N.D
0.15
0.06
0.067
N.D.
N.D.
0.2
0.15
pCO2
 
 
 
-5
 
 
 
-4.9
S.I.Calcite
 
 
 
0.2
 
 
 
0.4
S.I.Dolomite
 
 
 
<-3
 
 
 
<-3
S.I.Siderite
 
 
 
<-3
 
 
 
<-3
S.I.Quartz
 
 
 
0.3
 
 
 
0.1
S.I.Gibbsite
 
 
 
-0.2
 
 
 
-0.9
S.I.Gypsum
 
 
 
-2.6
 
 
 
-1
S.I.Barite
 
 
 
-2.1
 
 
 
-0.3
S.I.FeS
 
 
 
-0.3
 
 
 
-0.2
S.I.Fluorite
 
 
 
-1.1
 
 
 
0.9
                 

 
 

Sample
Ar
H2
He
O2
N2
CH4
C2H6
C3H8
iso-C4
n-C4
CO2
LEL
 
KL441FW050201XC56HWDS hole 2
1.68
< d.l.
15.56
6.55
61.91
23.35
0.51
0.05
< d.l.
< d.l.
< d.l.
5.20
 
KL443FW030501HWND 1
1.39
< d.l.
18.24
1.24
13.26
57.33
2.03
0.28
0.01
0.04
< d.l.
5.12
 
KL443FW050801XC43HWDN
1.79
< d.l.
20.82
0.97
16.80
53.43
2.04
0.21
0.01
0.02
< d.l.
5.12
 
   
 
 
 
 
     
KL441FW1C56HWDS- Dec-98
n.a.
< d.l.
10.70
7.21
58.10
22.30
0.48
0.04
< d.l.
< d.l.
n.a.
5.20
 
Notes:
< d.l. = below detection limit
LEL = Lower Explosive Limit
n.a. = not analyzed

References:

van Heerden, A.W.C., A Lithological and Geomorphological Study of the VCR Sand Facies observed within the 4-Shaft Complex, Kloof Gold Mine.
    Unpublished Report.


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